Phospho flow antibodies

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August undefined, 2024

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human. WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well.

Anti-Phosphoserine antibody (ab9332) Abcam

WebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. Share WebThis Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. Product Usage Information Storage dark purple stone with sparkles

BD™ Phosflow Protocol for Human PBMCs - BD Biosciences

WebJan 1, 2012 · Antibodies: 5. Anti-human and mouse phospho-STAT3 (pY705) mouse antibody conjugated to phycoerythrin (PE) (BD/Phosflow, catalogue number: 612569). 6. Anti-human and mouse phospho-STAT5a,b (pY694) mouse antibody conjugated to Alexa Fluor ® 488 (BD/Phosflow, catalogue number: 612598). 7. WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. dark purple small bathroom

How To Do Phospho-Flow Cytometry - Cheeky Scientist

Anti-NFAT1 (phospho S54) antibody (ab200819) Abcam

WebAll lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with … WebPhospho-SMC1A-S957 Polyclonal Antibody dark purple sleeveless one shoulder topsWebPrimary Anti-ULK1 (phospho S556) antibody (ab203207) Primary Anti-AMPK gamma 1 antibody [Y308] (ab32508) Primary Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) View more … dark purple spots on arms

"WebPhospho-NFkB p65 (Ser536) Antibody Applications: WB, FCM, ICC, IP Reactivity: Hu, Ms, Rt, Ca, Hm, NHPr, Pg Conjugate/Tag: Unconjugated Quantity: 100 µL Citations: (25) Supplier Page Sign In or Register to view pricing Compare Product + See More (4) View All (18) Biorbyt NFKB p65 antibody Applications: WB, ELISA, FCM, ICC, IF, IHC-fr, IHC-p " - Phospho flow antibodies

Phospho flow antibodies

Human and Mouse Phospho-PLCG2 (Tyr753) ELISA RayBiotech

WebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. WebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis …

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WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl … WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody.

WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material.

WebPhospho-S6 (Ser235, Ser236) Antibody (12-9007-42) in Flow. Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human phospho-S6 (S235/S236) PE, using the Intracellular Fixation and ... WebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728.

WebPhosphoserine Antibodies Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits …

WebPhospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb detects endogenous levels of Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. Species Reactivity: Human, Mouse, Rat Source / Purification bishop oudemanWebAnti-Phosphoserine antibody See all Phosphoserine primary antibodies Description Rabbit polyclonal to Phosphoserine Host species Rabbit Specificity Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. dark purple slim fit men shirt button downWebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is … dark purple sheer curtainsWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 … dark purple stud earringsWebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies … bishop o\u0027gorman mascotWebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: SMAD2, SMAD3; MADH2, MADH3; MAD homolog 2, MAD homolog 3 Reactivity: … bishop o\u0027hara high schoolWebPhospho-specific antibody flow cytometric analysis of phosphorylated proteins allows researchers a quick and effective way to measure signaling cascades in individual cells. A … bishop outdoor living