Hoechst 33342 toxicity

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August undefined, 2024

NettetDisplaying low-cytotoxic effects and increased cell permeability, Hoechst stains are ideal for live- and fixed-cell fluorescent DNA staining and nuclei imaging techniques, such as cell counting, automated DNA determination, cell cycle analysis, chromosome sorting, and nuclear counterstaining. Nettet15. sep. 2024 · hoechst 33342染色步骤. 1. 将荧光染料（Hoechst 33342、AO、PI 或 EB）以合适浓度直接添加到细胞培养基中。. 2. 将细胞孵育 5 分钟。. 3. 在倒置荧光显微镜下直接观察细胞。. 如果需要，在对转染细胞进行重要染色后修复细胞，以进一步详细分析形态：. 1.用PBS清洗细胞 ...

碧云天-C1022-Hoechst 33342(Hoechst 33842荧光染料)

Nettet10. aug. 2016 · Combining Lipinski’s rule with the docking and steered molecular dynamics simulations and using the PubChem data base of about 1.4 million compounds, we have obtained DNA dyes Hoechst 34580 and Hoechst 33342 as top-leads for the Alzheimer’s disease. The binding properties of these ligands to amyloid beta (Aβ) fibril were … Nettet28. jul. 2024 · Red Vybrant Tongue (V22885; Thermo Fisher Scientific Orange Red) was used to stain entire cells and Hoechst 33342 (H3570; Thermo Fisher Scientific) (3:1000) was used to stain nuclei. Following 10 minutes of staining, CurcuEmulsomes were added to the culture and images were taken in series for 15 minutes from different Z-stacks … black stainless switch plates

Measuring DNA content in live cells by fluorescence microscopy

NettetI'm planning to stain fixed Vero nuclei with Hoechst 33342. The protocol manual provides quite a range for both concentration (0.2 - 2 µg/ml) and incubation time (1 - 15 min) when staining... Nettet1. jan. 2009 · Hoechst 33342 is moderately water-soluble (up to 2% solutions can be prepared) and relatively non-toxic. The difference between the two bisbenzimides, Hoechst 33342 and Cellular toxicity of Hoechst 33342 Cell types differ with respect to their cytotoxic and kinetic responses to Hoechst 33342. NettetHoechst dyes are generally preferred for live cell staining over DAPI because they are less toxic and more cell permeant. Biotium offers both Hoechst 33342 and Hoechst … gary justice washington georgia

Breaking a Dogma: High‐Throughput Live‐Cell Imaging in …

Nettet13. okt. 2024 · Hoechst 33342 is a cell-membrane permeant dye that reacts exclusively with double-stranded DNA, while Pyronin Y, also a cell-membrane permeant dye, reacts with DNA, RNA, and mitochondrial membranes. In the presence of Hoechst 33342, the Pyronin Y reaction with DNA is blocked and predominantly stains RNA when used at … Nettet14. jan. 2024 · Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. Properties Articles 72 Spectrum Names Hoechst 33342 Biological Activity Chemical & Physical Properties MSDS Hoechst 33342 MSDS (Chinese) Toxicological Information Safety Information Synthetic Route Previous 1/3 Next … gary justice marion ncNettetIn this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in … black stainless switch cover

"Nettet1. jan. 2009 · Hoechst 33342 is moderately water-soluble (up to 2% solutions can be prepared) and relatively non-toxic. The difference between the two bisbenzimides, Hoechst 33342 and 33258, is that 33342 has an ethyl group instead of a … " - Hoechst 33342 toxicity

Hoechst 33342 toxicity

Nettet18. nov. 2024 · Hoechst 33342 (referred to as Hoechst) is a bis-benzamide dye that is relatively non-toxic and cell-permeable, making it suitable for a wide range of … NettetReproductive toxicity and gender differences induced by cadmium telluride quantum dots in aninvertebrate model organism. SCI REP-UK 2016 Sep 27;6:34182. (IF 3.998) 133. Xie W, Chen X, Miao G, Tang J, Fu X. Regulation of cellular behaviors ... Hoechst 33342活细胞染色液(100X) C1018.

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NettetToxicity and safety [ edit] Because Hoechst stains bind to DNA, they interfere with DNA replication during cell division. Consequently, they are potentially mutagenic and … NettetToxicity Toxic, harmful Health Hazards Wear PPE at all times, handle with glove. Potential Hazards CNS and Liver; harmful by skin absorption, irritant mutagen. Carcinogenicity: No significant acute toxicological data identified AnaSpec Inc. 34801 Campus Drive, Fremont, CA 94555 Toll-Free: 800-452-5530 • Tel: 510-791-9560 • Fax: …

Nettet15. des. 2024 · Labeling Nuclear DNA with Hoechst 33342 [1] Step 1, Dilute the Hoechst stock solution 1:100 in H 2 O for use in labeling. Step 2, Aspirate the cell medium from … Nettet18. jun. 2024 · The procedure was successfully tested for the analysis of cytotoxicity. The method is suitable for the quantification of cells exhibiting low metabolic activity including senescent cells. The...

NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye —blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient —provided as an easy-to-use Hoechst dye solution (20 mM) • Cellular imaging —cell-permeable dye is effective for fixed-cell and live-cell staining. • Counterstain —ideal for use ... NettetIn experiments on three leukemic bone marrows, H33342 was shown to be more toxic than DAPI in terms of colony-forming capability. Although there is considerable …

Nettet1. okt. 2010 · In this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at …

Nettet28. jan. 2014 · The kinetics of cell loss and the rather large dose modification factor suggests that Hoechst 33342 may be suppressing an apoptotic response in this subpopulation. Whatever the mechanism involved, Hoechst 33342 clearly provides substantial protection against early radiation-induced endothelial cell loss. gary justis lawyerNettetPage 2/6 Safety Data Sheet acc. to OSHA HCS Printing date 01/14/2024 Revision date 01/14/2024 Trade name: Hoechst 33342 (hydrochloride) (Contd. from page 1) gary justiceNettet20. feb. 2015 · The chapter also includes references on the use, properties, and safety/toxicity of Hoechst 33342 on humans, animals and the environment. It presents … gary justis law firmNettet30. mai 2010 · The intracellular accumulation of the fluorescent probe Hoechst (H) 33342 (bisbenzimide) by MAR mutants and those with defined disruptions in efflux pump and porin genes was determined in 96-well plate format. Results gary justice houstonNettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … black stainless teapotNettetChemical structure for Hoechst 33342 *Ultrapure Grade* *CAS 23491-52-3* Figure 7. Dose-dependent toxicity of diverse NPs towards HUVECs as measured by CDS assay. The HUVECs in the 96-well plate were treated with 0–100 μg/ml of SiNP, SPION or CNTCOOH NPs for 24 hours. black stainless tealleNettetfor 1 dag siden · Chlorpromazine, nystatin, and wortmannin solution were added to fibroblasts and incubated for 30 min followed by CSRBnps and incubated. At 30 min, 1, 4, and 12h exposure time, the CSRBnps were removed, and cells stained with Hoechst 33342 (NucBlue™ Live ReadyProbes™ Reagent, Invitrogen, USA)) in phenol red free … gary justice architect